Validation of multiplex PCR for detection and differentiation of Salmonellas

Salmonellosis is the zoonotic disease caused by Salmonella bacteria. These are food-borne pathogens, which require improvement of diagnostics and surveillance measures. Prior to implementation of a PCR-based system for monitoring Salmonella, presence and differentiation of the agent was validated under Office International Epizootical (O.I.E.) requirements.

Objective

This study aimed to perform interlaboratory testing and clarification of the PCR-based test for its implementation in Ukraine.