Pakistan

Karachi is the largest metropolitan, principal port city and commercial hub of Pakistan. Although there is a national database for registering vital evens such as births and deaths but like in any other developing country the coverage of the system is sub optimal with many of birth and death not recorded. Undercounting of these events leads to inaccurate estimates of vital indicators for informed decision and planning for health at local and national level. In these settings demographic surveillance systems have the potential to supplement data from the vital registration systems and provide avenues for research by virtue of having a well-defined cohort and continuous surveillance. The Department of Paediatrics and Child Health of Aga Khan University Karachi, Pakistan maintains health and demographic surveillance system at four peri-urban and one urban community in Karachi with focus on maternal and child health. This also provide platform for many epidemiological and interventional studies as well as vaccination programs using a well-established identification procedures providing linkage to health and socio-economic data. In 2010 the surveillance system was reorganized to follow INDEPTH methodology and guidelines.

Objective

The purpose of Karachi Health and Demographic Surveillance System (HDSS) is to generate longitudinal information on health and demographics of a geographically defined population of low socioeconomic status and provide platform for larger projects in efforts for diseases control.

Livestock sector contributes more than 58% to agriculture-based economy of Pakistan. Diseases of socio-economic importance are posing an enormous pressure to the growth of this sector. Zoonotic diseases are generally neglected in wake of epizootics having epidemic potential. One Health is a multi-sectoral approach to control zoonotic diseases at animal level to mitigate risk of transfer to the humans and environment. Despite various control programs, zoonosis is known to cause public health emergencies at various regional and national levels. OIE declared brucellosis as a model bacterial disease to control zoonosis in developing countries. Genus Brucella is expanding with its discovery in various amphibian species and marine mammals and demands control efforts at various levels. Reporting of zoonosis is less than actual prevalence in third world countries like Pakistan where disease is considered endemic but no official data is available. In this study, brucellosis was used as a model disease to emphasize the significance of One Health.

Objective:

To detect the presence of brucella in serum samples of occupationally exposed human and animals by conventional screening methods. To perform epidemiosurvelliance of brucella molecular based tests including genus and species specific PCR. To check the brucella prevalence in occupationally exposed human.