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Elaboration of diagnostic means for detection antibodies to Newcastle disease virus

Description

Newcastle disease (ND) is the most important infectious viral disease of poultry. The world-wide economic loss from it is 2-3 billion USD per year. ND is reportable to the World Organization for Animal Health (OIE). ND is caused by virulent strains of avian Paramyxoviruses belonging to type 1. Industrial poultry farming is rapidly developing in Ukraine. Ornithological fauna of Ukraine includes about four hundred species of birds, 207 of which nest within its borders. The territory of Ukraine transits 3 out of 14 transcontinental global migration flows. The wild birds are the main natural reservoir of ND agents. It is necessary to control the intensity of post-vaccination immunity in poultry and the timing of revaccinations. OIE recommends enzyme linked immunosorbent assays (ELISA) and HI test for these purposes. However, it should be noted that HI test, possessing high specificity and sensitivity, is much cheaper. Therefore, it is the excellent means for ND timely surveillance.

Objective

A test kit for the detection of antibodies to Newcastle disease virus (NDV) based on haemagglutination inhibition (HI) assay has been developed and introduced into practice for the first time in Ukraine.

Submitted by teresa.hamby@d… on