African swine fever virus (ASFV), pseudorabies virus (Aujrszky’s disease virus, ADV) and porcine circovirus type 2 (PCV-2) are some of the most dangerous DNA viruses causing high amounts of morbidity and mortality in commercial and backyard pig farms. Traditional diagnosis of porcine viral infections requires complex and lengthy combinations of virological and serological tests. This study aimed to develop a method for rapid detection of the DNA-containing viral pathogens of pig in clinical materials using conventional multiplex PCR platform.
Objective
The objective was to develope the multiplex PCR for detection of the DNA-contained emergent diseases agents in pigs (African swine fever, Aujeszky disease, Circoviral disease) for diseases surveilance in pig farms