Nepal being a developing country has many health problems, which escalate in intensity at different times of the year or in epidemic form. Amebiasis is one of the infectious diseases that is highly seen in rural area of Nepal caused by Entamoeba species. Recent reports show that open defecation, drinking untreated water, unsanitary habits and lack of basic health knowledge cause higher mortality and morbidity in our country. E. histolytica is an anaerobic pathogenic parasitic. However, E. dispar and E.moshkovskii exits as non-pathogenic. Likewise, E. histolytica, E. dispar and E. moshkovskii are morphologically identical but genetically distinct species.
Objective: To find out the prevalence of Entamoeba species in rural community of Nepal. The purpose of the study is to evaluate Nested PCR, microscopic examination and Elisa assay for detection and differentiation of Entamoeba species.